Anything uPick (Endgame)

[Taly]

Ily all of you 38

[Cheeky Wings]

39

[Taly]

Nancy am I OK 39

[Taly]

OH WAIT 40

AND NOW 41

[Ircher]

Experiment is tomorrow but presentation is Friday I mean

What are you presenting? 42

[jjh927]

Taly are you capable of counting

43

[Ircher]

44

[Cheeky Wings]

Wake me up when something interesting

less depressing

happens.

45

[Taly]

I'm presenting my progress to the lab

I'm trying to induce 2 HP1 gene mutations into the fly genome through a gene-editing technique called Homology Directed Repair that requires a conserved sequence of the actual wild-type (Normal) gene so that we hope our mutation is repaired into the genome through recombination. This is after an endonucleade known as Cas9 cuts the double stranded DNA of the HP1 gene to allow for the repair repair to occur.

And I'm currently modifying and replicating a plasmid DNA (bacterial) from a strain of E. Coli to try and create our mutant DNA that contains abconserved sequence but the mutation.

I'm also then doing just typical fly experiments regarding the HP1C protein specifically. I'm taking flies with either a completely absent HP1C protein or ineffective but present HP1C protein and seeing how that affects fly Fertility and Hatch Rate to determine the organisms fitness in development.

And then I'm drafting a grant for RNA-sequencing for the fly work above.

46

[Taly]

Taly are you capable of counting

43

Im capable enough to recall this shit

47

Nosebleed stopped bye lovelies I love you all

[Taly]

People are like "tAlY yoR so SmArT"

And I'm like girl I just want a man to pick me up and take me away from the world.i want want men on men on me so

48

Ok actually bye now

[Ircher]

Ooh fun stuff.

49

[jjh927]

50

We are now a tenth of the way to the objective

[Good Luck Buddy]

51 i forgot what the objective was
how is everyone doin tonite

[jjh927]

52

Is there anyone who hasn't said they scumread you at some point, Fire?

[Good Luck Buddy]

u know what. i can't even recall.
i think pooky got like 10 people to do it

[Good Luck Buddy]

and i got 0 to scumread me.
embarassing

[Ircher]

53 embarrassing indeed.

[Good Luck Buddy]

54 so hows school going ircher

[Ircher]

55

pretty good for the most part. I have a major presentation tomorrow which is really the only thing that worries me.

[Cheeky Wings]

Are you introducing the manipulated genes into germ cells? Sounds like crispr.
~C

[Cheeky Wings]

I'm debating whether or not to Google this fly gene.
56
~C

[Cheeky Wings]

So in terms of anti spew which is scummier? Posting anime or counting to 500.or being T3 rn.
~C

[jjh927]

57

[Ircher]

五十九